Takashi Horiuchi
Tokai University, Japan
Title: Double rolling circle replication (DRCR): a mode of amplification of oncogene as well as drug-resistant genes and replication of HSV and chloroplast DNA
Biography
Biography: Takashi Horiuchi
Abstract
It is well established that eukaryote nuclear chromosomes are duplicated from multiple origins of replication. It remains a mystery, however, how genomes of some viruses, such as HSV (Herpes simplex virus) and Baculovirus, or chroloplasts, are replicated. We found recently that (i) double rolling circle replication (DRCR), originally found responsible for replication of yeast 2 micron plasmid DNA, can lead to amplification of oncogenes as well as drug resistance genes, and (ii) that DRCR is highly recombinogenic. In addition, we will present our model, based on these findings, that DRCR is involved in DNA replication of HSV-1, chloroplasts and some mitochondria. The model could explain how DRCR contributes to replication-recombination coupling of HSV, and also how it promote amplicon shortening during gene amplification. HO induced